Journal: Nature Metabolism

Article Title: Skeletal muscle from TBC1D4 p.Arg684Ter variant carriers is severely insulin resistant but exhibits normal metabolic responses during exercise

doi: 10.1038/s42255-024-01153-1

Figure Lengend Snippet: a , Graphical representation of the EHC. b , GIR during the insulin clamp. c – e , GIR ( c ), endogenous glucose rate of appearance (RA) ( d ) and glucose rate of disappearance (RD) ( e ) at 120 min of the clamp. f , Plasma FAs during the clamp. g , Average leg glucose uptake during the last 40 min of the clamp. h , Targeted immunoblotting analyses of proteins associated with glucose metabolism, fat metabolism and the mitochondrial electron transport chain in skeletal muscle. i , Volcano plot showing proteome log 2 fold change (FC) (carriers/controls) plotted against –log 10 P value highlighting downregulated proteins TBC1D4, TBC1D1 and GLUT4 (SLC2A4). *Difference between TBC1D4 carriers and controls at the given time point. ‡Different from basal (time 0) within the same group. n = 5 ( b – g , i ). n = 7 ( h ) except for mTOR, p70S6K and ACC ( n = 5 in controls and TBC1D4 carriers) as well as Akt2, AMPKα2, CS, CD36 and FATP4 ( n = 6) in controls and GLUT1 ( n = 6) in TBC1D4 carriers. Data are means ± s.e.m. Data were analysed using a two-tailed paired Student’s t -test ( c – e , g ), a two-tailed non-paired Student’s t -test ( h ) as well as a two-way repeated ANOVA test (two-factor repeated) and two-tailed Student–Newman–Keuls post hoc analyses for multiple comparisons ( b , f ). Graphics in a created using BioRender.com . a.u., arbitrary units.

Article Snippet: For staining, four to six individual fibres were teased out from the fixed fibre bundles with fine forceps and blocked for 2 h in PBS containing 5% goat serum, 1% BSA and 0.04% saponin and then incubated overnight with primary antibody raised against GLUT4 (rabbit polyclonal; no. PA5-23052; lot WJ3403509, Thermo Scientific).

Techniques: Western Blot, Two Tailed Test

Journal: Nature Metabolism

Article Title: Skeletal muscle from TBC1D4 p.Arg684Ter variant carriers is severely insulin resistant but exhibits normal metabolic responses during exercise

doi: 10.1038/s42255-024-01153-1

Figure Lengend Snippet: a , Representative immunoblots for data related to figure panel 2 h. b , c , Distribution of Myosin Heavy Chains (MHC) ( b ) and regulatory metabolic ( c ) proteins in MHC-defined type 1 and type 2 skeletal muscle fiber bundles. d , Representative immunoblots. e , Quantification of GLUT4 imaging by confocal microscopy of isolated skeletal muscle fibers. n = 7 in Controls and n = 6 in TBC1D4 carriers ( b , c ). n = 15 fibers obtained from 3 subjects (4 to 6 fibers/subject) in each group ( e ). Data are means ± SEM. Data were analyzed using a two-tailed non-paired students t-test ( e ) as well as a two-way repeated ANOVA test (one factor repeated) and two-tailed Student-Newman-Keuls post hoc analyses for multiple comparisons ( b , c ). AU, arbitrary units. Scale bar in panel e is 10 µm.

Article Snippet: For staining, four to six individual fibres were teased out from the fixed fibre bundles with fine forceps and blocked for 2 h in PBS containing 5% goat serum, 1% BSA and 0.04% saponin and then incubated overnight with primary antibody raised against GLUT4 (rabbit polyclonal; no. PA5-23052; lot WJ3403509, Thermo Scientific).

Techniques: Western Blot, Imaging, Confocal Microscopy, Isolation, Two Tailed Test

Journal: Research

Article Title: A Wearable Integrated Microneedle Electrode Patch for Exercise Management in Diabetes

doi: 10.34133/research.0508

Figure Lengend Snippet: Statistical analysis of dynamic variation of C glu and the closed-loop feedback mode for diabetes management. (A) Analytical model for evaluating the dynamic variation of C glu in terms of 8 parameters. (B) Statistical analysis of 8 parameters obtained from control and experimental groups designated as G1 to G8 ( n = 6 rats). (C) Fluorescence images of Alexa-488-conjugated WGA and Alexa-594-stained GLUT4 of skeletal muscle sections from type 2 diabetic model rats after resting, walking, jogging, and fast running. (D) t -SNE for G1 to G8. The 4 ellipses indicate the confidence ellipse (95% confidence interval). (E) Flow block diagram of the closed-loop feedback mode for diabetes management.

Article Snippet: They were first incubated with rabbit polyclonal antibody against GLUT4 (Thermo Fisher Scientific, PA5-23052; antibody was diluted in 1× PBS to 10 μg ml −1 ) overnight at 4 °C, followed by 10 min of washing for 3 times, and the second incubation for 1 h with goat anti-rabbit F(ab′) 2 fragment-specific antibody conjugated to Alexa-594 (Jackson ImmunoResearch, 111-585-047; antibody was diluted in 1× PBS to 3.75 μg ml −1 ).

Techniques: Control, Fluorescence, Staining, Blocking Assay

Journal: International journal of molecular sciences

Article Title: Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells.

doi: 10.3390/ijms25105155

Figure Lengend Snippet: Figure 2. Effects of AdipoRon on the transport of glucose in rat ovarian granulosa cells. (A) GLUT1, GLUT2, GLUT3, and GLUT4 protein levels after 24 h of the in vitro addition of different concentrations

Article Snippet: The goat polyclonal anti-rabbit AdipoR2 (sc-46751, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse AdipoR1 (sc46748, Santa Cruz Biotechnology, Dallas, TX, USA), goat polyclonal anti-rabbit GLUT1 (21829-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT2 (20436-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal antirabbit GLUT3 (20403-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT4 (sc-53566, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse vinculin (sc-73614, Santa Cruz Biotechnology, Dallas, TX, USA), and rabbit polyclonal anti-mouse adiponectin (ab181281, abcam Biotechnology, Cambridge, UK) were also used.

Techniques: In Vitro

Journal: International journal of molecular sciences

Article Title: Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells.

doi: 10.3390/ijms25105155

Figure Lengend Snippet: Figure 4. Transcriptome data and correlation analysis of the expression of glucose transporters and the adipokine system in rat ovaries. (A) Volcanic map analysis of transcriptome data. Blue: represents no significant difference. (B) KEGG pathway analysis; (C) correlation analysis of genes related to the glucose metabolism signaling pathway and adipokine signaling pathway. (D) Gene transcription levels of Slc2a1, Slc2a2, Slc2a3, Slc2a4, Adipor1, and Adipor2 in the ovaries of rats in the eCG-treated and blank control groups. (E) Protein levels of GLUT1, GLUT2, GLUT3, GLUT4, AdipoR1, and AdipoR2 in the ovaries of rats in the eCG-treated and blank control groups. The error bars represent means ± SEM (n = 3, each group). * Statistically significant values (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001); ns represents no significance.

Article Snippet: The goat polyclonal anti-rabbit AdipoR2 (sc-46751, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse AdipoR1 (sc46748, Santa Cruz Biotechnology, Dallas, TX, USA), goat polyclonal anti-rabbit GLUT1 (21829-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT2 (20436-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal antirabbit GLUT3 (20403-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT4 (sc-53566, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse vinculin (sc-73614, Santa Cruz Biotechnology, Dallas, TX, USA), and rabbit polyclonal anti-mouse adiponectin (ab181281, abcam Biotechnology, Cambridge, UK) were also used.

Techniques: Expressing, Control